人抗中性粒細胞細胞質PR3/MPO IgG檢測試劑盒說明書

人抗中性粒細胞細胞質PR3/MPO IgG檢測試劑盒

Human ANCA Screen (Anti-PR3 and Anti-MPO) IgG ELISA kit

cat#3300-250-ANG

Intended Use

This is a qualitative enzyme immunoassay (EIA) intended to screen for the presence of IgG class autoantibodies against PR3 and MPOin human serum or plasma. For in vitro research use only.

Principle

A mixture of highly purified PR3 and MPO antigens is bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bindto the respective antigens. After washing and addition of Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the boundpatient antibodies forming a conjugate/antibody/antigen complex. Washing and addition of enzyme substrate in the presence of bound conjugatehydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measuredphotometrically at 450nm.The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

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